2010 - TTS International Congress


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Laboratory Immunology and Kidney Transplantation

102.4 - Expression of E-cadherin, HSP(Heat Shock Protein)47, TGF-β1 and C4d in Chronic Allograft Nephropathy

Presenter: Samuel, Lee, Seoul, Korea
Authors: Lee S., Chung Y., Park J., Kim D., Park S., Kim J., Hyun S., Nam E.

EXPRESSION OF E-CADHERIN, HSP(HEAT SHOCK PROTEIN)47, TGF-?1 AND C4D IN CHRONIC ALLOGRAFT NEPHROPATHY

LABORATORY IMMUNOLOGY AND KIDNEY TRANSPLANTATION

S. Lee1, Y. Chung2, J. Park2, D. Kim2, S. Park1, J. Kim1, S. Hyun1, E. Nam1
1, Kangdong Sacred Heart Hospital, Hallym University Medical Center, Seoul/KOREA, 2, Kangdong Sacred Heart Hospital, Hallym University Medical Center, seoul/KOREA

Body:
Introduction; Chronic allograft nephropathy(CAN ) causing graft failure is related with tubular atrophy and interstitial fibrosis. E-cadherin is well known epithelial marker and HSP47 is acollagen-specific molecular chaperon and regulates collagen synthesis. TGF- β1, a profibrotic cytokine, downregulates E-cadherin and induces the expression of mesenchymal markers in the vitromodel. The expression of C4d is considered as a poor prognostic marker of graft survival.
Purpose; This study is aimed to evaluate the relationship between the expression of E-cadherin, HSP47, TGF-β1 or C4d and the prognosis in CAN.
Methods; Between March, 1991 and August, 2007, we performed renal allograft biopsies from 42 recipients with deteriorating renal function. CAN was diagnosed according to the chronic allograft damageindex(Banff classification, 1997). Renal allograft biopsies were examined for the expression of E-cadherin, HSP47, TGF-β1 or C4d by immunohistochemistry. The immunohistochemical studies wereperformed with the immunoperoxidase technique using the primary antibody: rabbit anti-human TGF- β1 polyclonal antibody (C-teminus, 1:1000, Santa-Cruz Biotechnology, Santa Cruz, CA, USA) , mouseanti-human Hsp 47(heat shock protein 47) monoclonal antibody (clone M16.10A1, 1:100, Stressgen, Ann Arbor, MI, USA), mouse anti-human E-Cadherin monoclonal antibody ( clone SPM471, 1:100, Lab Vision,Fremont, CA, USA) and mouse anti-human C4d monoclonal antibody (1:200, Nevus Biological Inc, Littleton, Col). The staining of Hsp47, TGF- β1 or E-cadherin was scored semiquantitatively using agrading from to 3 (0: <5%, 1: 5ï‚£ and < 25%, 2: 25%ï‚£ and <50%, 3: >50%) by analyzing ten different fields of cortical interstitium and tubules on highpower fields(x200). Biopsies with circumferential endothelial C4d staining in peri-tubular capillaries(25%) were designated as C4d-positive.
Result; Of 42 recipients, graft survival group(Gs) was 17(40.5%), graft failure group(Gf), 25(59.5%). The expression of E-cadherin in tubular cells in Gs was much higher than Gf(94.1% vs 52%,p=0.04). The expression of HSP47 in tubular cells and interstitium in Gf was much higher than Gs(84% vs 35.3%, p=0.001). The expression of TGF-ï¢1 in tubular cells and interstitium in Gfwas much higher than Gs(72% vs 23.5%, p=0.02).
Conclusion; The expression of E-cadherin, HSP47, TGF-β1 was strongly correlated with the prognosis in CAN.

Disclosure: All authors have declared no conflicts of interest.


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