2011 - IPITA - Prague


Parallel session 3 – Open oral presentations Topic: Stem cells

3.4 - Bone marrow stem cells and islet co-transplantation promotes graft revascularization and function in small intestinal submucosa

Presenter: E., Berishvili, Tbilisi, Georgia
Authors: E. Berishvili, Z. Kakabadze, I. Amiranashvili, K. Chutkerashvili



Bone marrow stem cells and islet co-transplantation promotes graft revascularization and function in small intestinal submucosa

E. Berishvili, Z. Kakabadze, I. Amiranashvili, K. Chutkerashvili
Tbilisi State Medical University, Tbilisi, Georgia

Objective: Type 1 diabetes is associated with aprogressive loss of beta cells and islet cell transplantation represents one ofthe most promising therapies. Recently we have shown that small intestinalsubmucosa is a sutable place for pancreatic islet transplantation. In thisstudy we show that transplantation of bone marrow cells into the smallintestinal submucosa allows minimal islet mass improve glycemic control in mousemodel.

Methods: Segments of small intestine were prepared by denudationof the mucosal layer prior to implantation. Streptozotocin induced diabetic C57Bl/6mice were transplanted syngeneically into the small intestinal submucosa withthe following: 100 islets alone (islet group: n = 12), 100 islets and 5 × 106bone marrow cells (islet-bone marrow group: n = 12) and sham operated group nocells (sham group: n = 5). Blood glucose levels were monitored andintraperitoneal glucose tolerance tests carried out. Histological assessment forinsulin, glucagon, von Willebrand factor (vWF) was performed.

Results: Co-transplantation of 100 islets and 5 × 106bone marrow cells reversed diabetes in all recipients, whereas islet-alonetransplantation achieved euglycemia in 2 of 12 recipients. Transplanted isletsdemonstrated expression of insulin and glucagon throughout the 90-day durationof the studies. Results of intravenous glucose tolerance tests performed on day56 were significantly better in islet-bone marrow group than islet-alonerecipients. One week after transplantation, well-preserved islet structure andhigher number of capillaries were found in the small intestinal segments ofislet- bone marrow recipients, whereas islet-alone grafts were fragmented withvery few capillaries. Removal of graft-bearing intestinal segments led torecurrence of hyperglycemia.

Conclusions: Islet co-transplantation with bone marrow isassociated with improvement of islet graft function.


You must be logged in to view recordings

Important Disclaimer

By viewing the material on this site you understand and accept that:

  1. The opinions and statements expressed on this site reflect the views of the author or authors and do not necessarily reflect those of The Transplantation Society and/or its Sections.
  2. The hosting of material on The Transplantation Society site does not signify endorsement of this material by The Transplantation Society and/or its Sections.
  3. The material is solely for educational purposes for qualified health care professionals.
  4. The Transplantation Society and/or its Sections are not liable for any decision made or action taken based on the information contained in the material on this site.
  5. The information cannot be used as a substitute for professional care.
  6. The information does not represent a standard of care.
  7. No physician-patient relationship is being established.

Our Corporate Sponsors

TTS gratefully acknowledges the Corporate Partners whose generous support makes the work of the Society possible:

  • astellas
  • roche
  • sanofi