2011 - ISBTS 2011 Symposium


Oral Communications 6: Surgical Aspects of ITX

8.146 - Autologous anorectal transplantation in the rat: manometrical evaluation

Presenter: Flavio, Galvao, SAO PAULO, Brazil
Authors: Victor Seid1, Flavio Galvao1, Daniel Waisberg1, Andre Lee1, Raoni Galvao1, Eleazar Chaib1, Luiz D'Albuquerque1


146
Autologous anorectal transplantation in the rat: manometrical evaluation

Victor Seid, Flavio Galvao, Daniel Waisberg, Andre Lee, Raoni Galvao, Eleazar Chaib, Luiz D'Albuquerque

Department of Gastroenterology, University of Sao Paulo Medical School, Sao Paulo, Brazil

Introduction: Anorectal transplantation is a potential proposal for severe anorectal dysfunction. The lacking of experimental models hampers the development of this technique.

Objective: To evaluate anorectal function in a model of autologous anorectal transplantation in rats.

Methods: Sixty male Wistar rats were divided in 4 groups (n=15): autologous ortothopic anorectal transplantation, autologous heterothopic transplantation, sham and normal rat. Body weight measurement, clinical evaluation and manometry were performed pre-operatively and on 2nd, 7th and 14th post-operative day (POD). Material for histopathological analysis was collected following sacrifice on 14th POD.

Results: Surgical procedure period was around 40 minutes. Ortothopic and heterothopic groups showed satisfactory evolution; however, animals from group I achieved faster recovery, higher body mass gain, normalization of clinical parameters on 7th POD and higher survival rate (86.7%). In group II animals, scores were numerically higher until study end point, with 66.6% survival rate [Final body mass: 319.5 ± 40.7g (ortothopic); 292.7 ± 38.3g (heterothopic); 317.8 ± 36.6g (sham); 322.9 ± 34.1g (control); p<0.01. Final behavior scores: 0.3 ± 0.5 (ortothopic); 0.6 ± 0.7 (heterothopic); 0.2 ± 0.4 (sham); 0.1 ± 0.3 (control); p<0.01. Final stool scores: 0.1 ± 0.4 (ortothopic); 0.3 ± 0.5 (heterothopic); 0.0 ± 0.0 (sham and control); p=0.01. Final stoma scores: 0.0 ±0.0 (ortothopic. sham and control); 0.7 ± 1.0 (heterothopic); p<0.01]. All surviving animals showed adequate sphincter structure in histopathological analysis. Manometry on study end point, ortothopic and sham groups showed numerically similar values compared to pre-operative data and statistically similar values to control group (ortothopic 25,4±18,2, sham 25,3±4,2, control 30,4±18,2 cmH20, p>0.05). Heterothopic group showed final values lower than their pre-operative data or control group (10,5±10,5 cmH20, p=0,01).

Conclusion: We described a feasible model of autologous anorectal transplantation in rats to study anorectal function recovery. Ortothopic group showed better clinical and eletromanometrical evolution than heterothopic group.


You must be logged in to view recordings

Important Disclaimer

By viewing the material on this site you understand and accept that:

  1. The opinions and statements expressed on this site reflect the views of the author or authors and do not necessarily reflect those of The Transplantation Society and/or its Sections.
  2. The hosting of material on The Transplantation Society site does not signify endorsement of this material by The Transplantation Society and/or its Sections.
  3. The material is solely for educational purposes for qualified health care professionals.
  4. The Transplantation Society and/or its Sections are not liable for any decision made or action taken based on the information contained in the material on this site.
  5. The information cannot be used as a substitute for professional care.
  6. The information does not represent a standard of care.
  7. No physician-patient relationship is being established.

Our Corporate Sponsors

TTS gratefully acknowledges the Corporate Partners whose generous support makes the work of the Society possible:

  • astellas
  • roche
  • sanofi