2011 - ISBTS 2011 Symposium


Oral Communications 6: Surgical Aspects of ITX

8.148 - A simple novel technique for heterotopic intestinal transplantation in mice

Presenter: Xiao-Sun, Liu, Hangzhou, People's Republic of China
Authors: Xiao-Sun Liu1, Peng-Hong Song1, Shu-Yang Zhou1, Jiong Tian1, Sheng Yan1, Ji-Ren Yu1, Zhou-Ke Tan2, Song Rong2,3


148
A simple novel technique for heterotopic intestinal transplantation in mice

Xiao-Sun Liu1, Peng-Hong Song1, Shu-Yang Zhou1, Jiong Tian1, Sheng Yan1, Ji-Ren Yu1, Zhou-Ke Tan2, Song Rong2,3

1Department of General Surgery, the First Affiliated Hospital, College of Medcine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China; 2Transplantation Center, Affiliated Hospital, Zunyi Medical College, Zunyi, Guizhou, People's Republic of China; 3Department of Nephrology, Hannover Medical School, Hannover, Germany

Background: The murine heterotopic intestinal transplant model is of particular value for addressing the unique immune responsein the gut, and addressing the underlying immune mechanism during transplantation. Anastomotic stenosis, anastomotic bleeding and septic shock continue to hamper procedures and plague the success.

Methods: In this study, we assessed a refined technique designed to improve the success of HIT in mice. Important factors in our refined technique included (i) a refined procedure for graft portal vein preparation, (ii) a novel procedure for graft exteriorization and most importantly (iii) a knotless suturing technique designed to allow the surgeon to alter the size of the anastomosis, thus reducing anastomotic bleeding and stenosis rate.

Results: Our refined knotless method improved recipient survival to 67.5% when compared to the standard technique (53.8%).In comparison to standard knot suturing technique, which had an anastomotic stenosis rate of 8.3%, the knotless suturing technique significantly reduced the rate of anastomotic stenosis to only 2.4%.Anastomotic bleeding presented in the knotless technique in only 1.2%, whereas it presented in 6.2%, of mice in the standard technique (p < 0.05).

Conclusion: This refined knotless technique offers an easy and effective method for murine heterotopic intestinal transplantation. 

List grant support: The project was supported by the National Natural Science Foundation of China (Grant 30860277) and Zhejiang Provincial Natural Science Foundation of China (Grant Y2110019).


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