2011 - CTS-IXA


This page contains exclusive content for the member of the following sections: TTS, CTS, IXA. Log in to view.

Parallel Session 20- Encapsulation (Cell Track)

35.530 - Optimization of nano-scale emulsions: perfluoro micellar solutions for enhanced oxygen transfer in biomedical applications

Presenter: Christopher, Fraker, Miami, United States
Authors: Christopher A. Fraker1,2, Kerim Gattas-Asfura1, Armando Mendez1,3,5, Luca Inverardi1,3,4, Camillo Ricordi1,2,3, Cherie L. Stabler1,2

530

Optimization of nano-scale emulsions: perfluoro micellar solutions for enhanced oxygen transfer in biomedical applications

Christopher A. Fraker1,2, Kerim Gattas-Asfura1, Armando Mendez1,3,5, Luca Inverardi1,3,4, Camillo Ricordi1,2,3, Cherie L. Stabler1,2

1Biomedical Engineering; 2Department of Surgery; 3School of Medicine; 4Microbiology and Immunology; 5Division of Endocrinology, Diabetes and Metabolism, University of Miami, Miami, FL, United States

 

Nano–scale emulsification has long been utilized by the food and cosmetics industry to maximize material delivery through increased surface area to volume ratios. More recently, these methods have been employed in the area of biomedical research to enhance and control the delivery of numerous pharmaceutical and cytoprotective compounds. Of particular interest is the use of perfluorocarbon (PFC) nano-scale emulsions to increase overall oxygen mass transfer within the surrounding milieu. In this work, we developed and implemented methods for optimizing the manufacture of stable emulsions focusing on component selection, emulsification time, emulsification pressure and accurate characterization of temporal emulsion stability and oxygen delivering capacity. Through careful analysis of these parameters we were able to design reproducible and well-defined oxygen-delivering PFC emulsions for use in cell-based applications.

Oxygen mass transfer, or diffusive permeability, is determined by the product of the effective oxygen diffusivity through and oxygen solubility in the emulsion. We found that particle size was the critical factor affecting oxygen mass transfer, as increased micelle size resulted in reduced oxygen diffusion, canceling the benefit of increased dissolved oxygen content in the perfluorocarbon phase of the emulsion. Particle size stability was directly related to the perfluorocarbon utilized, particularly the molecular weight, diffusivity through the liquid phase and interfacial tension between the hydrophobic and hydrophilic emulsion components. Overall, this work demonstrated the importance of accurate characterization of emulsification parameters in order to generate stable, reproducible emulsions with the desired bio-delivery properties.


Important Disclaimer

By viewing the material on this site you understand and accept that:

  1. The opinions and statements expressed on this site reflect the views of the author or authors and do not necessarily reflect those of The Transplantation Society and/or its Sections.
  2. The hosting of material on The Transplantation Society site does not signify endorsement of this material by The Transplantation Society and/or its Sections.
  3. The material is solely for educational purposes for qualified health care professionals.
  4. The Transplantation Society and/or its Sections are not liable for any decision made or action taken based on the information contained in the material on this site.
  5. The information cannot be used as a substitute for professional care.
  6. The information does not represent a standard of care.
  7. No physician-patient relationship is being established.

Social

Contact

Staff Directory
+1-514-874-1717
info@tts.org

Address

The Transplantation Society
International Headquarters
740 Notre-Dame Ouest
Suite 1245
Montréal, QC, H3C 3X6
Canada