2013 - CTS 2013 Congress

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Oral Communications 8

16.6 - Betain supplement enhances skeletal muscle differentiation in murine myoblasts via IGF-1 signaling activation

Presenter: Ileana , Terruzzi, Milan, Italy
Authors: Pamela Senesi^1,2,3, Livio Luzi^1,2,3, Anna Montesano^1,2,3, Nausicaa Mazzocchi^1,2,3, lleana Terruzzi^1,2,3

Betain supplement enhances skeletal muscle differentiation in murine myoblasts via IGF-1 signaling activation

Pamela Senesi^1,2,3, Livio Luzi^1,2,3, Anna Montesano^1,2,3, Nausicaa Mazzocchi^1,2,3, lleana Terruzzi^1,2,3

¹Department of Biomedical Sciences for Health , University of Milan, Milan, Italy; ²Research Centre, San Donato Hospital and Scientific Institute, San Donato Hospital and Scientific Institute, Milan, Italy; ³Nutrition-Metabolism Unit, San Raffaele Scientific Institute, Milan, Italy

Betaine (BET) is a component of many food, including spinach and wheat. It is an essential osmolyte and a source of methyl groups. Recent studies have hypothesized that BET might play a role on athleticperformance. However, BET effects on skeletal muscle differentiation and hypertrophy remain not completely known.

In this study, we examined BET action on neo myotubes maturation and myogenesis, using C2C12 murine myoblastic cells. By dose-response study, we determined that 10 mM BET was the dose able to stimulate morphological changes and hypertrophic process in neo myotubes. Using RT²-PCR array strategy to identify the expression profile of genes, encoding proteins involved in IGF-1 pathway, we found that 10 mM BET could promote IGF-1 receptor (IGF-1 R) expression. Western blot and immunofluorescence analysis pointed out that, in neo myotubes, 10 mM BET improved not only IGF-1 signaling, but also the synthesis of Myosin Heavy Chain (MyHC), the major skeletal muscle marker. Moreover, 10 mM BET increased neo myotubes length.

In addition, we investigated BET role on myoblasts proliferation and differentiation. During proliferation, BET did not modify C2C12 proliferative rate, but promotedmyogenic induction, enhancing MyoD protein content and cellular elongation. During differentiation, BET raised muscle-specific markers and IGF-1 R protein levels.

These findings provide the first evidence that BET could promote muscle differentiation and myotubes size by IGF-1 pathway activation, indicating that BET might represent a possible new drug/integrator strategy, not only in sport performance but also in clinical conditions characterized by muscle function injury.

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