PERFUSION BY OXYGENATED BUFFER CONTAINING PROSTAGLANDIN E1 PRIOR TO COLD PRESERVATION PREVENTS WARM ISCHEMIA-REPERFUSION INJURY IN LIVER GRAFTS FROM NON-HEART-BEATING DONORS

LATE BREAKING I

Y. Hara¹, Y. Akamatsu¹, Y. Kobayashi², T. Iwane¹, S. Satomi^3
1Division Of Advanced Surgical Science And Technology, Tohoku university, Sendai/JAPAN, ²Advanced Surgical Science And Technology Division, Department Of Surgery, Tohoku Univarsity hospital, Sendai/JAPAN, ³Division Of Advanced Surgical Science And Technology, Tohoku University, Sendai/JAPAN

Body: Introduction: We have previously reported that perfusion by oxygenated warm buffer prior to cold preservation (pre-perfusion) improved the viability and function of liver grafts from non-heart-beating donors (NHBDs) by restoring energy charge and ATP content in liver tissue and reducing apoptotic cell death. The aim of this study was to evaluate various mitochondrial functions on pre-perfusion as well as the effect of addition of prostaglandin E1 (PGE1) to pre-perfusion. Methods: Rat livers were harvested after 30 minutes warm ischemia and perfused for one hour after cold preservation in UW solution for 6 hours (NHB group). Livers in pre-perfusion group (PRE group) were perfused for 30 minutes before cold preservation by oxygenated Krebs-Henseleit buffer at 37 ^oC. In PG group, PGE1 (10ng/ml) added to pre-perfusion buffer. Mitochondria were extracted from livers after one hour reperfusion freshly and applied to functional analysis. Results: Portal flow and bile production in PRE and PG groups were higher than that in NHB group. In particular bile production in PG group was significantly increased. ALT and TNF-α in the perfusate significantly decreased in PG group. Mitochondrial proton ATPase activity tended to be higher in PRE and PG groups compared to NHB group. In PG group, mitochondrial swelling was significantly suppressed and total amount of cytochrome c in mitochondria was significantly maintained. Table1.Poral Flow and Bile production During Reperfusion, ALT and TNF-α in the Perfusate ; Data were shown as the mean ± SD.(n=5).*P<0.05 vs NHB

Groups	Portal Flow(ml/g/h)	Bile Production(μL/g/h)	ALT(Karmen uits)	TNF-α(pg/ml)
NHB	211±30.0	24±3.8	4.2±0.69	22.1±3.94
PRE	237±21.6	30±2.5	3.6±0.94	21.5±3.23
PG	263±35.5	35±5.9*	2.6±0.31*	9.8±6.97*

Conclusion: Perfusion by oxygenated buffer containing PGE1 prior to cold preservation significantly protected mitochondrial function, suppressed the release of cytochrome c from mitochondria and alleviated various cellular disorders in the livers suffered warm ischemia-reperfusion injury. This method is suggested as a promising strategy for liver transplantation from NHBDs.

Disclosure: All authors have declared no conflicts of interest.