2011 - IPITA - Prague


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Parallel session 6 – Open mini-oral presentations Topic: Clinical and experimental islet transplantation

6.9 - Systemic effects of liver regeneration on rat beta cells and islets: In vivo and in vitro studies

Presenter: F., Moreau, STRASBOURG, France
Authors: F. Moreau, F. Toti, S. Sigrist, W. Bietigier, E. Seyfritz, M. Pinget, L. Kessler


Systemic effects of liver regeneration on rat beta cells and islets: In vivo and in vitro studies

F. Moreau1, F. Toti2, S. Sigrist3, W. Bietigier3, E. Seyfritz3, M. Pinget1, L. Kessler1
1 University hospital and Faculty of Medicine of University of Strasbourg, STRASBOURG, France; 2 Hematology and Immunology Institut, Strasbourg, France; 3 Cellular and Genetic Laboratory, Centre Européen d'Etude du Diabète, Strasbourg, France

Background: Liver regeneration induces the release of humoral factors in situ and in blood circulation possibly contributing to pancreatic islet engraftment. This work studies the systemic effect of liver regeneration on the native pancreas in normal rats and in vitro by examining the outcome of ratRINm5F beta cells, grown in standard medium supplemented with normal or diabetic hepatectomized (Hx) rat serum.
Material and methods: 2/3 partial hepatectomy was performed in normal and streptozotocin induced diabetic Lewis rats (n=6). Pancreas from control, SHAM or Hx normal rats were weighted one, two or three days after surgery, islets size examined by insulin staining. RINm5F ß cells were grown in RPMI medium containing 10% serum from Hx rats. After 24 h, cell viability and count were evaluated by CellTiter and cell counter.
Results: The ratio pancreas/body weight was higher in Hx rats compared to SHAM,3 days after surgery: 0.58 vs 0.39 (p=0,057). Proportion of small size islets(<10 000 µm2) increased significantly from 67% to 92% (p<0.05). By comparison with the serum obtained from Sham animals at day two or three after surgery, Hx normal rat serum promoted a significant increase inRinm5F cell viability: 126% vs 94% (p=0,005) 121% vs 94% (p=0,006) respectively and cell proliferation measured at day 3 increased accordingly : 4,62 vs 3,57 10 5 cells / ml (p=0,036). Conversely, Hx diabetic rat serum withdrawn 3 days after hepatectomy had an opposite effect with significant decrease in cell viability: 79 vs 95% (p=0,036).

Conclusion: Liver regeneration induces systemic effects on pancreatic islets and increases their viability in normal rat but not in diabetic rat. Further studies with assessments of islet functionality will be achieved on islets isolated 72h after partial hepatectomy.


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