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Presenter: J., Escartín, Zaragoza, Spain
Authors: F.A. García-Gil, C.D. Albendea, L. Fuentes-Broto, J. Rosello-Catafau, E. Folch-Puy, V. Bernal, E. Piedrafita, L. López-Pingarrón, J. Escartín, J.J. García
IGL-1 preservation solution prevents lipidperoxidation and changes in membrane fluidity during pancreas allotransplant in pigs
F.A. García-Gil1, C.D. Albendea2, L. Fuentes-Broto2, J. Rosello-Catafau3, E. Folch-Puy3, V. Bernal4, E. Piedrafita2, L. López-Pingarrón2, J. Escartín1, J.J. García2
1 University of Zaragoza, Surgery, Zaragoza, Spain; 2 University of Zaragoza, Department of Pharmacology and Physiology, Zaragoza, Spain; 3 Institut d'Investigacions Biomèdiques de Barcelona (IIBB), Consejo Superior de Investigaciones Científicas, Barcelona, Spain; 4 University Hospital San Jorge, Gastroenterology and Hepatology , Huesca, Spain
Objective: Over the last few years, the Institute Georges Lopez preservation solution (IGL-1) was introduced for the perfusion and cold storage of abdominal grafts. IGL-1 is an extracellular-type electrolyte solution that contains polyethylene glycol 35 as a colloid. Up to now no data have been published as to the effects of IGL-1 in pancreas transplantation (PT). We evaluated the efficacy of IGL-1 in experimental PT, assessing additionally the effects on graft oxidative stress.
Methods: Sixteen Landrace pigs underwent allogenic PT with enteric drainage of
exocrine secretions and systemic venous drainage, with 16 hours of cold ischemia. Grafts were preserved with IGL-1 (n=8) or Wisconsin solution (UW) (n=8). No immunosuppresion was administered. PT endocrine and exocrine function was evaluated. Pancreatic graft samples were collected during procurement prior to vascular clamping (basal), after 16 h of cold storage, and 30 min after vascular reperfusion. Membrane fluidity was monitored by fluorescence spectroscopy. Malondialdehyde (MDA) and 4-hydroxy-alkenals (4-HDA) were measured as an index of lipidperoxidation.
Results: All grafts with IGL-1, but only 6 (75%) with UW were functioning (at least 5 days of normoglycemie). Graft failures with UW were an arterial thrombosis the first day and a venous thrombosis the fourth day. With IGL-1, no differences were found between membrane fluidity basal and post-reperfusion levels (2.789 and 2.780 1/P respectively, P>0.05). Lower levels than basal membrane fluidity values were found with UW after reperfusion. (2.730 and 2.612 1/P respectively, P<0.05). Lipidperoxidation after reperfusion with IGL-1 was lower than with UW (0.218 and 0.318 nmol MDA+4-HDA/mg protein respectively, P<0.05).
Conclusions: Our results indicate that IGL-1 is effective in pig PT with 16 hours of cold ischemia. IGL-1 prevents changes in membrane fluidity and decreases lipidperoxidation in the grafts after reperfusion. Furthermore, it seems likely that IGL-1 may be useful to prevent pancreatic graft vascular thrombosis.
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