2013 - ISBTS 2013 Symposium

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Posters and Exhibition

15.55 - Analysis of isolated lymphoid follicles after intestinal transplantation

Presenter: Dominik, Meier, , Argentina
Authors: Dominik Meier1, Guillermo Docena2, Diego Ramisch3, Juan Padin3, Ulises Toscanini4, Gabriela Berardi4, Gabriel E. Gondolesi1,3, Martín Rumbo2

Analysis of isolated lymphoid follicles after intestinal transplantation

Dominik Meier1, Guillermo Docena2, Diego Ramisch3, Juan Padin3, Ulises Toscanini4, Gabriela Berardi4, Gabriel E. Gondolesi1,3, Martín Rumbo2

1Laboratorio de Investigación Traslacional e Inmunología de Trasplante, Universidad Favaloro, Buenos Aires, Argentina; 2Laboratorio de Investigaciones del Sistema Inmune , Universidad Nacional de La Plata, La Plata, Argentina; 3Instituto de Trasplante Multiorgánico, Hospital Universitario Fundación Favaloro, Buenos Aires, Argentina; 4PRICAI, Hospital Universitario Fundación Favaloro, Buenos Aires, Argentina

 

Isolated lymphoid follicles (ILF), located in the gut mucosa, are highly dynamic structures serving as an inductive site for IgA synthesis confronting changes in the gut microbiota. Mature ILF contain germinal center where rapid proliferation of B cells and immunoglobulin class switching take place. No studies have been conducted about the behavior of ILF in intestinal allograft tissue. Therefore, we wanted to elucidate immune status and turnover of donor-receptor cells within allograft ILF after transplantation.
     We compared ILF from non-transplanted, non-pathogenic distal ileum tissue (n=11) with allograft ILF from ileostomy closure tissue (n=8) by flow cytometry, quantitative real time PCR, and immunohistology. Short tandem repeat (STR) technology was used to calculate the percentage of donor-receptor ratio within ILF captured by laser dissection microscopy of 6-days post-transplant biopsies and tissues from ileostomy closure.
     We found that allograft ILF from ileostomy closure tissue express 7-fold increased level of activation-induced cytidine deaminase mRNA, master regulator for immunoglobulin class switch, than non-transplant ILF. On paraffin section, the Ki-67 proliferation marker revealed a higher number of positive cells in the allograft ILF samples compared to sections from non-transplant tissue. A 1.4-fold higher percentage of CD8 T cells were detected in allograft ILF, whereas expression of the activation marker CD69 on T cells was comparable in allograft versus non-transplant ILF. The STR analysis revealed that at 6 days post-operation 50% of allograft ILF tissue was still of donor origin, whereas at the ileostomy closure around one year post-transplant, around 95% of the tissue was of recipient origin.
     In conclusion, allograft ILF have a higher level of activity than non-transplanted ILF. We speculate that changing in microbiota might be one of the reasons for this difference in allograft ILF compared to non-transplant ILF. Our results open the path for a better understanding of allograft mucosal immunity.

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