2013 - ISBTS 2013 Symposium
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Posters and Exhibition
15.71 - Inbred and outbred rat model of orthotopic small bowel transplantation.
Presenter: Necdet, Ozcay, , Turkey
Authors: Necdet Ozcay1, Gokhan Moray1, Mehmet Haberal1
Inbred and outbred rat model of orthotopic small bowel transplantation.
Necdet Ozcay1, Gokhan Moray1, Mehmet Haberal1
1General Surgery, Baskent University, Ankara, Turkey
Although significant improvements of graft and patient survival have been achieved in the field of intestinal transplantation, the results are far from ideal especially for long term survival. Therefore intestinal transplantation continues to be performed only in situations in which all other therapeutic modalities have failed. We developed a rat model of small bowel transplantation to study these difficulties seen after intestinal transplantation.
Donor operation: All the donors and recipients are anesthetized with an intraperitoneal injection of ketamine (50 mg/kg), supplemented with xylasine (10 mg/kg). The abdomen is opened via a midline incision. The entire colon is removed and the portal vein is separated from the pancreas , pyloric and splenic veins are ligated. The superior mesenteric artery with a long segment of aorta is mobilized. After the graft is perfused in-situ with cold heparinized lactated ringer’s solution, the intestine and its vascular supply are removed en-bloc and stored in lactated ringer’s solution.
Recipient operation: The recipient’s infrarenal aorta and inferior vena cava are mobilized. A modified Lee’s clamp is placed across these vessels. Longitudinal arteriotomy and venotomy are made and anastomoses are performed by using a continuous 10-0 nylon sutures.The clamp is slowly released after completion of the anastomoses. The recipient’s jejunum and ileum are removed leaving only 2 cm each of the native jejunum and ileum. The intestinal anastomoses are performed using one layer full thickness 7-0 silk continuous suture. The animal’s recieve 10 ml/hour of lactated Ringer’s solution by intermittent injection in the dorsal penile vein.
Outbred Sprague-Dawley rats are ideal for practising microsurgical procedures because they are resistant and inexpensive. Inbred Sprague-Dawley rats are perfect for physiology studies since they are isogenic and have a very kind behavior. We are going to use these models to study intestinal transplantation in our labaratory.
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