P-227 Poster of distinction

The cytokine response in the initial phase of islet auto- and allo-transplantation

C. Pollard¹, W.Y. Chung¹, C. Drogemuller², C. Milner², M.A. Webb¹, S. Illouz¹, G. Gravante¹, T. Radford², D. Al-Leswas¹, P.T. Coates², G. Maddern³, A.R. Dennison^1
1 University Hospitals of Leiceter NHS Trust, Leicester, U.K.; ² Royal Adelaide Hospital, Adelaide, Australia; ³ The Queen Elizabeth Hospital, Adelaide, Australia

Objectives: Clinical islet transplantation is now recognised as an important procedure for the treatment of Type 1 diabetes and results continue to improve. It is also increasingly being used to prevent the onset of diabetes in patients who have undergone pancreatectomy for the treatment of chronic pancreatitis. Islet auto- and allo- transplantation trigger an acute inflammatory response and may cause significant loss and degradation of islets. Affected patients may require transplantation of further islet as a consequence. There are many reports examining the inflammatory response in islet transplantation, but there are no direct comparisons of the initial inflammatory phase following islet auto- and allo- transplantation. This study aimed to compare the cytokine profiles of these two groups.

Methods: Blood samples were collected during induction of anaesthesia, at the end of the surgical reconstruction, end of operation, 1 hour, 3 hours, 5 hours and days 1-7 postoperatively from two control (pancreatectomy without an islet transplant), three islet auto-transplant (Leicester General Hospital) and two islet allo-transplant (Royal Adelaide Hospital, Australia) patients. Multiplex enzyme-linked immunoassays were used to measure cytokines levels. Two way ANOVA was used for statistic analyses of islet auto and allo transplantation.

Results: INF?, TNF?, and IL-10 were significantly increased in islet allo-transplant patients compared to those receiving an islet auto-transplant (p<0.0001, p<0.0087 and p<0.0066 respectively). IL-6 increased in the control group and islet transplantation after surgery. IL-1?, IL-2 and GM-CSF do increase after islet infusion, but there is no statistically significant different between islet auto and allo transplantation.

Conclusions: The cytokine profile following islet transplantation suggests a significantly greater acute inflammatory response following allo-transplantation compared to auto-transplantation.

P-227 Poster of distinction

The cytokine response in the initial phase of islet auto- and allo-transplantation

C. Pollard¹, W.Y. Chung¹, C. Drogemuller², C. Milner², M.A. Webb¹, S. Illouz¹, G. Gravante¹, T. Radford², D. Al-Leswas¹, P.T. Coates², G. Maddern³, A.R. Dennison^1
1 University Hospitals of Leiceter NHS Trust, Leicester, U.K.; ² Royal Adelaide Hospital, Adelaide, Australia; ³ The Queen Elizabeth Hospital, Adelaide, Australia

Objectives: Clinical islet transplantation is now recognised as an important procedure for the treatment of Type 1 diabetes and results continue to improve. It is also increasingly being used to prevent the onset of diabetes in patients who have undergone pancreatectomy for the treatment of chronic pancreatitis. Islet auto- and allo- transplantation trigger an acute inflammatory response and may cause significant loss and degradation of islets. Affected patients may require transplantation of further islet as a consequence. There are many reports examining the inflammatory response in islet transplantation, but there are no direct comparisons of the initial inflammatory phase following islet auto- and allo- transplantation. This study aimed to compare the cytokine profiles of these two groups.

Methods: Blood samples were collected during induction of anaesthesia, at the end of the surgical reconstruction, end of operation, 1 hour, 3 hours, 5 hours and days 1-7 postoperatively from two control (pancreatectomy without an islet transplant), three islet auto-transplant (Leicester General Hospital) and two islet allo-transplant (Royal Adelaide Hospital, Australia) patients. Multiplex enzyme-linked immunoassays were used to measure cytokines levels. Two way ANOVA was used for statistic analyses of islet auto and allo transplantation.

Results: INF?, TNF?, and IL-10 were significantly increased in islet allo-transplant patients compared to those receiving an islet auto-transplant (p<0.0001, p<0.0087 and p<0.0066 respectively). IL-6 increased in the control group and islet transplantation after surgery. IL-1?, IL-2 and GM-CSF do increase after islet infusion, but there is no statistically significant different between islet auto and allo transplantation.

Conclusions: The cytokine profile following islet transplantation suggests a significantly greater acute inflammatory response following allo-transplantation compared to auto-transplantation.