P-240

Improved the effect of islet transplantation by endothelial cells coating and infusion Sertoli cells

Y. Li¹, W. Xue¹, X. Tian¹, X. Ding¹, X. Feng¹, P. Tian¹, J. Hou¹, X. Pan¹, H. Yan¹, H. Xiang¹, C. Ding¹, S. Li^2
1 Renal Transplant Department,Center of Nephropathy,The First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an, China; ² Forensic Medicine, Medical College, Xi'an Jiaotong University, Xi'an, China

Background: Improving islet vascularization and inhibiting rejection become key points for prolonging islet graft survival. Endothelial cells (ECs) are the basis of islet vascularization and Sertoli cells have the effects nutritional support and immunosuppression. We construct vascularized islet by a method of ECs coating to cnstruct vascularized islet. Sertoli cells were intravenous infused to inhibit rejection in islet transplantation. Thereby, the survival rate could be improved by a decrease of islet graft loss. Methods: Islets, ECs and Sertoli cells were isolation from SD rats. ECs and islet were co-cultured in culture tube for 2 hr and then transferred to heparin treated petri dish. After successful coated, streptozotocin-induced diabetic rats were divided into three groups before islet transplantation: single islet and ECs coating islets were transplanted in group A and B respectively; group C with Sertoli cells infusion and ECs coating islets transplantation. The meansurvival time (MST), insulin expression and microvessel density (MVD) of islet grafts were measured. The number of lymphocytes and the levels of cytokines in peripheral blood were also measured.

Results: Group C had the longest MST of islet allografts (59.8 7.81 days) followed by groups B(21.3 6.22 days) and A(11.0 4.47 days) (P<0.05).Immunohistochemistry showed similar results with MST. Microvessel density (MVD) of group C (17.2 2.6) were significantly higher than those in group A(1.6 1.1) and B(8.0 1.5)(P<0.05). The rats in group C had the least CD4+T cells (only18.1 6.1%) compared with other two groups (P<0.05). The numbers of CD8+ T cells in rats of groups C (12.2 5.3%) were significantly lower than those of groups A and B (P<0.05).

Conclusion: The program of vascularization by ECs coating islet and immune tolerance induction could effectively prolong the survival of islet grafts.

P-240

Improved the effect of islet transplantation by endothelial cells coating and infusion Sertoli cells

Y. Li¹, W. Xue¹, X. Tian¹, X. Ding¹, X. Feng¹, P. Tian¹, J. Hou¹, X. Pan¹, H. Yan¹, H. Xiang¹, C. Ding¹, S. Li^2
1 Renal Transplant Department,Center of Nephropathy,The First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an, China; ² Forensic Medicine, Medical College, Xi'an Jiaotong University, Xi'an, China

Background: Improving islet vascularization and inhibiting rejection become key points for prolonging islet graft survival. Endothelial cells (ECs) are the basis of islet vascularization and Sertoli cells have the effects nutritional support and immunosuppression. We construct vascularized islet by a method of ECs coating to cnstruct vascularized islet. Sertoli cells were intravenous infused to inhibit rejection in islet transplantation. Thereby, the survival rate could be improved by a decrease of islet graft loss. Methods: Islets, ECs and Sertoli cells were isolation from SD rats. ECs and islet were co-cultured in culture tube for 2 hr and then transferred to heparin treated petri dish. After successful coated, streptozotocin-induced diabetic rats were divided into three groups before islet transplantation: single islet and ECs coating islets were transplanted in group A and B respectively; group C with Sertoli cells infusion and ECs coating islets transplantation. The meansurvival time (MST), insulin expression and microvessel density (MVD) of islet grafts were measured. The number of lymphocytes and the levels of cytokines in peripheral blood were also measured.

Results: Group C had the longest MST of islet allografts (59.8 7.81 days) followed by groups B(21.3 6.22 days) and A(11.0 4.47 days) (P<0.05).Immunohistochemistry showed similar results with MST. Microvessel density (MVD) of group C (17.2 2.6) were significantly higher than those in group A(1.6 1.1) and B(8.0 1.5)(P<0.05). The rats in group C had the least CD4+T cells (only18.1 6.1%) compared with other two groups (P<0.05). The numbers of CD8+ T cells in rats of groups C (12.2 5.3%) were significantly lower than those of groups A and B (P<0.05).

Conclusion: The program of vascularization by ECs coating islet and immune tolerance induction could effectively prolong the survival of islet grafts.