Ischemic preconditioning attenuates the inflammatory response In rats after intestinal ischemia/reperfusion

Daniel FC Pinheiro, Belchor Fontes, John K Shimazaki, Paola Vendramini, Sonia M Silva, Joilson O Martins, Jacqueline F Jacysyn, EDNA FS MONTERO

Introduction. The intestinal ischemia occurs in many disease processes, resulting from the occlusion of the superior mesenteric artery to low flow states such as hemorrhagic shock. Ischemia / reperfusion (I/R) can lead to intestinal mucosal injury and trigger the release of various inflammatory mediators, resulting in a systemic inflammatory response syndrome that leads to multiple organ damage, in particular acute lung injury, and finally to the dysfunction and failure of multiple organs and systems.

Objective. The objective of this research project was to evaluate the effect of ischemic preconditioning (IPC) and to determine the inflammatory response resulting from intestinal ischemia and reperfusion (I/R) in rats.

Methods. It was used male Wistar rats, free from diseases, weight between 250 and 350 grams, from the Central Animal Laboratory. The animals were divided into two groups: I/R and IPC Groups. Rats have been submitted to ischemic preconditioning (IPC) by occluding superior mesenteric artery (10 minutes followed by 10 minutes of reperfusion), immediately before the 45 minutes of sustained ischemia and two hours of reperfusion. It was collected lung samples to analyze CINC-1 and -2,  determined through ELISA, as well as MDA nM/mg of protein.

Results. CINC-1 and CINC-2 levels decreased in the IPC group compared to I/R group (CINC-1: I/R=0.81 to IPC=0.67; CINC-2: I/R=0.52 to IPC=0.35; p<0.05). MDA values were lower in IPC group (0.62nM/mg of protein) than I/R group (1.23nM/mg of protein; p<0.05).

Conclusion. Ischemic preconditioning atenuates intestinal ischemia reperfusion injury, decreasing inflammatory response.