2016 - IPITA - Stem Cell Derived Beta Cells Workshop

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Genetic Modification and Genome Editing of Pluripotent Stem Cells

6.3 - Human pancreas development and disease through the lens of pluripotent stem cells

Presenter: Danwei, Huangfu, New York, United States
Authors: Danwei Huangfu

My laboratory is interested in understanding pancreatic development and disease through applying genetic approaches in human pluripotent stem cells (hPSCs), including embryonic and induced pluripotent stem cells (hESCs and hiPSCs). Combining CRISPR/Cas-mediated gene editing and stem cell technologies, our reverse genetics approach has revealed the roles of key transcription factors pancreatic development and diabetes. Using the forward genetics approach, we performed the first genome-wide knockout screen for identification of developmental regulators. We identified novel genes that regulate the formation of definitive endoderm cells, which give rise to most pancreatic cells among other cells in the respiratory and digestive tract. Together our findings establish the use of hPSCs as a genetic model system for studying congenital defects and diabetes.

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