P-189

Islet transplantation in the rat: analysis of implantation sites correlated to islet survival rate

F.Y. Matsushita, E. Chaib, B.B. Varone, F.Y. Hirata, G.R. de Melo, L.A.C. D’ Albuquerque

Liver Transplantation Surgery Unit and LIM-37, University of São Paulo, School of Medicine, São Paulo, Brazil

Background: Islets can be isolated and transplanted into different sites with the aim to normalize blood glucose in diabetic rats.

Aim: The purpose of this work is to discover which transplant site has the longer ISR (Islet Survival Rate) and better capacity to normalize blood glucose levels in diabetics rats.

Method: Through online research using PubMed and SCIELO we have selected papers from 1996 to 2010, concerning islets transplantation and its methods. We have included only experiments with rats and excluded all the other rodents. The data were compiled into a table.

Results: The islet survival rate (ISR) according to different sites of implantation were: Peritoneal Cavity: ISR- 10,8 days, Liver: ISR-107,96 days, Intramuscular: ISR-100 days, Spleen: ISR- 90 days, Kidney: ISR-68,46 days, Omental Pouch: ISR- 56 days, Seminal Vesicles:ISR- 42 days, Peritoneal Cavity (Immunosupressed rats): ISR- 18+-8 days.

Conclusion: Although there were many sites to implant the islets, the best place to implant it seems to be the peritoneal cavity because the islet survival rate were longer than others.

Parallel poster session 3 - Topic: Alternate sources of insulin-producing cells

P-189

Islet transplantation in the rat: analysis of implantation sites correlated to islet survival rate

F.Y. Matsushita, E. Chaib, B.B. Varone, F.Y. Hirata, G.R. de Melo, L.A.C. D’ Albuquerque

Liver Transplantation Surgery Unit and LIM-37, University of São Paulo, School of Medicine, São Paulo, Brazil

Background: Islets can be isolated and transplanted into different sites with the aim to normalize blood glucose in diabetic rats.

Aim: The purpose of this work is to discover which transplant site has the longer ISR (Islet Survival Rate) and better capacity to normalize blood glucose levels in diabetics rats.

Method: Through online research using PubMed and SCIELO we have selected papers from 1996 to 2010, concerning islets transplantation and its methods. We have included only experiments with rats and excluded all the other rodents. The data were compiled into a table.

Results: The islet survival rate (ISR) according to different sites of implantation were: Peritoneal Cavity: ISR- 10,8 days, Liver: ISR-107,96 days, Intramuscular: ISR-100 days, Spleen: ISR- 90 days, Kidney: ISR-68,46 days, Omental Pouch: ISR- 56 days, Seminal Vesicles:ISR- 42 days, Peritoneal Cavity (Immunosupressed rats): ISR- 18+-8 days.

Conclusion: Although there were many sites to implant the islets, the best place to implant it seems to be the peritoneal cavity because the islet survival rate were longer than others.

Parallel poster session 3 - Topic: Alternate sources of insulin-producing cells