P-204

Expression of pancreatic endocrine markers by mesenchymal stem cells from human adipose tissue

A. Calil, A.L. França, C.L. Rebelatto, C.M. Aita
Pontifícia Universidade Católica do Paraná, Curitiba, Brazil

Mesenchymal stem cells (MSCs) from human adipose tissue have a great potential for use in cell therapy due to its ease of isolation, expansion and differentiation, besides relative acceptance of the ethical point of view.

Objective: Our intention was to isolate and promote in vitro expansion and differentiation of MSCs from human adipose tissue into cells with a pancreatic endocrine phenotype.

Methods: Human adipose tissue was obtained from patients undergoing abdominal dermolipectomy. Adipose tissue was digested with type I collagenase, MSCs were isolated by plastic adherence, characterized by cytochemistry and FACS and in vitro expanded. MSC differentiation into an endocrine phenotype was induced with high glucose (25 mmol/L) media during 2-4 months containing nicotinamide, exendin-4 and 2-mercaptoethanol. Insulin, somatostatin, glucagon and PDX-1 expression was analyzed by immunofluorescence.

Results: Cells isolated from the human adipose tissue and expanded in vitro expressed MSC markers as confirmed by FACS and cytochemistry. Insulin, glucagon and PDX-1 expression by differentiated cells were demonstrated by immunofluorescence.

Conclusions: MSCs isolated from the human adipose tissue were induced to differentiate in vitro into an endocrine phenotype and expressed insulin, glucagon and PDX-1.

P-204

Expression of pancreatic endocrine markers by mesenchymal stem cells from human adipose tissue

A. Calil, A.L. França, C.L. Rebelatto, C.M. Aita
Pontifícia Universidade Católica do Paraná, Curitiba, Brazil

Mesenchymal stem cells (MSCs) from human adipose tissue have a great potential for use in cell therapy due to its ease of isolation, expansion and differentiation, besides relative acceptance of the ethical point of view.

Objective: Our intention was to isolate and promote in vitro expansion and differentiation of MSCs from human adipose tissue into cells with a pancreatic endocrine phenotype.

Methods: Human adipose tissue was obtained from patients undergoing abdominal dermolipectomy. Adipose tissue was digested with type I collagenase, MSCs were isolated by plastic adherence, characterized by cytochemistry and FACS and in vitro expanded. MSC differentiation into an endocrine phenotype was induced with high glucose (25 mmol/L) media during 2-4 months containing nicotinamide, exendin-4 and 2-mercaptoethanol. Insulin, somatostatin, glucagon and PDX-1 expression was analyzed by immunofluorescence.

Results: Cells isolated from the human adipose tissue and expanded in vitro expressed MSC markers as confirmed by FACS and cytochemistry. Insulin, glucagon and PDX-1 expression by differentiated cells were demonstrated by immunofluorescence.

Conclusions: MSCs isolated from the human adipose tissue were induced to differentiate in vitro into an endocrine phenotype and expressed insulin, glucagon and PDX-1.