P-205

Differential glucose transporter expression in New World Primates (NWP) and in rodent pancreatic islets

D. Mohanasundaram¹, C. Drogemullar¹, T. Coates^2
1 Royal Adelaide Hospital, CNARTS, Adelaide, Australia; ² Department of Medicine, University of Adelaide, Adelaide, Australia

Objective: Glucose transporters are a family of membrane proteins found in most mammalian cells that transport the glucose to activate insulin release. They are structurally related proteins with distinct patterns of expression and biochemical properties. These transporters facilitate the induction of experimental diabetes using STZ. Aim of the present study is to investigate the expression and regulation of glucose transporter in new world primate islets compared to rodent islets for the induction of experimental diabetes prior to islet cell transplantation research.

Method: frozen section of pancreatic islets of NWP, rat and mice were processed for the detection of protein expression of Glucose transporter2 (GLUT2), Glucose transporter1 (GLUT2) and Glucokinase using immunofluorescence techniques with appropriate primary and secondary antibodies. Expression of these transporters at the messenger levels were carried out by Taqman PCR assay after checking the quality and quantity by Experion.

Results: the results on protein expression revealed that the expression GLUT2 was significantly low in NWP islets compared to rodent islets, where as in mRNA level the expression was detectable. In contrast, GLUT1 of NWP was expressed at the protein level but very low at the messenger level

Conclusion: These findings on glucose transporters regulation may be a cause for ineffective induction of diabetes in NWP. The findings on rodents will be helpful in the effective induction of experimental diabetes before islet cell transplantation and also to understand the mechanism of glucose sensing in these species in islet biology research.

P-205

Differential glucose transporter expression in New World Primates (NWP) and in rodent pancreatic islets

D. Mohanasundaram¹, C. Drogemullar¹, T. Coates^2
1 Royal Adelaide Hospital, CNARTS, Adelaide, Australia; ² Department of Medicine, University of Adelaide, Adelaide, Australia

Objective: Glucose transporters are a family of membrane proteins found in most mammalian cells that transport the glucose to activate insulin release. They are structurally related proteins with distinct patterns of expression and biochemical properties. These transporters facilitate the induction of experimental diabetes using STZ. Aim of the present study is to investigate the expression and regulation of glucose transporter in new world primate islets compared to rodent islets for the induction of experimental diabetes prior to islet cell transplantation research.

Method: frozen section of pancreatic islets of NWP, rat and mice were processed for the detection of protein expression of Glucose transporter2 (GLUT2), Glucose transporter1 (GLUT2) and Glucokinase using immunofluorescence techniques with appropriate primary and secondary antibodies. Expression of these transporters at the messenger levels were carried out by Taqman PCR assay after checking the quality and quantity by Experion.

Results: the results on protein expression revealed that the expression GLUT2 was significantly low in NWP islets compared to rodent islets, where as in mRNA level the expression was detectable. In contrast, GLUT1 of NWP was expressed at the protein level but very low at the messenger level

Conclusion: These findings on glucose transporters regulation may be a cause for ineffective induction of diabetes in NWP. The findings on rodents will be helpful in the effective induction of experimental diabetes before islet cell transplantation and also to understand the mechanism of glucose sensing in these species in islet biology research.