P-206

Clinical isolation and purification of fetal hematopoietic stem cells for treatment of diabetes mellitus

B. Arjmand, H.R. Aghayan, E. Nasli-Esfahani, F. Abbasi, B. Larijani
Tehran University of Medical Sciences, Endocrinology and Metabolism Research Center, Tehran, Iran

Background: Stem cell therapy is an attractive treatment modality in many fields of medicinesuch as diabetes mellitus.Fetal hematopoietic stem cells have some valuable advantages as pluri-potent properties and also there is no immunosuppression after transplantation. Iran has a leadership role in stem cell research among the Middle Eastern Muslim countries. Endocrinology and Metabolism Research Institute, has started working on isolation and purification of fetal stem cells in order to treat diabetes as clinical trials.

Fetal stem cell preparation: After obtaining informed consent, fetal liver is procured from legally therapeutic abortion. Donors are selected according to inclusion and exclusion criteria and maternal blood sample is tested for viral markers. Isolation and purification hematopoietic stem cells is performed under specific condition to maintain maximum safety and efficacy of cell product. A satellite Sample from the each batch is checked for microbial contamination, CFU-GEMM assay, Viability and phenotype (CD-markers). After cryopreservation stem cells are stored in liquid nitrogen until releasing for clinical application.

Conclusion: Fetal Hematopoietic stem cells are valuable sources for clinical transplantation in diabetes mellitus. Despite pluri-potent properties that make them powerful in renewing , they shows very low expression of MHC-I and also no expression of MHC-II on the cell surface, that can be transplanted without immunosuppression.

Keywords: Cell therapy . Diabetes mellitus . Fetal stem cell

P-206

Clinical isolation and purification of fetal hematopoietic stem cells for treatment of diabetes mellitus

B. Arjmand, H.R. Aghayan, E. Nasli-Esfahani, F. Abbasi, B. Larijani
Tehran University of Medical Sciences, Endocrinology and Metabolism Research Center, Tehran, Iran

Background: Stem cell therapy is an attractive treatment modality in many fields of medicinesuch as diabetes mellitus.Fetal hematopoietic stem cells have some valuable advantages as pluri-potent properties and also there is no immunosuppression after transplantation. Iran has a leadership role in stem cell research among the Middle Eastern Muslim countries. Endocrinology and Metabolism Research Institute, has started working on isolation and purification of fetal stem cells in order to treat diabetes as clinical trials.

Fetal stem cell preparation: After obtaining informed consent, fetal liver is procured from legally therapeutic abortion. Donors are selected according to inclusion and exclusion criteria and maternal blood sample is tested for viral markers. Isolation and purification hematopoietic stem cells is performed under specific condition to maintain maximum safety and efficacy of cell product. A satellite Sample from the each batch is checked for microbial contamination, CFU-GEMM assay, Viability and phenotype (CD-markers). After cryopreservation stem cells are stored in liquid nitrogen until releasing for clinical application.

Conclusion: Fetal Hematopoietic stem cells are valuable sources for clinical transplantation in diabetes mellitus. Despite pluri-potent properties that make them powerful in renewing , they shows very low expression of MHC-I and also no expression of MHC-II on the cell surface, that can be transplanted without immunosuppression.

Keywords: Cell therapy . Diabetes mellitus . Fetal stem cell