2011 - ISBTS 2011 Symposium

Oral Communications 2: Ischemia / Reperfusion

4.115 - Protective effect of ischemic postconditioning on the tissue ischemic injury following intestinal autotransplantation

Presenter: Andrea, Ferencz, Pecs, Hungary
Authors: Andrea Ferencz1, Gyorgy Weber1, Erzsebet Roth1, Klara Nedvig1

Protective effect of ischemic postconditioning on the tissue ischemic injury following intestinal autotransplantation

Andrea Ferencz, Gyorgy Weber, Erzsebet Roth, Klara Nedvig

University of Pecs, Pecs, Hungary

Ischemia/reperfusion injury is a grave condition resulting from intestinal transplantation. The aims of our work was to follow up the oxidative stress parameters and tissue structural changes during intestinal autotransplantation testing the protective effect of ischemic postconditioning (IPO) especially to investigate the activation of transcriptional factor Nuclear Factor-kappaB (NF-kB).

Total orthotopic autotransplantation was performed in Wistar rats (n=30). Grafts were stored in cold University of Wisconsin solution for 1, 3 and 6 hours. Reperfusion took 3 hours. Prior to reperfusion IPO was performed by 3 cycles of 30 second ischemia and 30 second reperfusion. Tissue damage was evaluated by Park’s and software Scion Image on HE-stained sections. To monitor oxidative stress concentration of tissue malondialdehyde (MDA) and reduced glutathione (GSH), and the activity of superoxide dismutase (SOD) were determined. Cytoplasmic and nuclear NF-kB level were detected after laparotomy (control), 30 minutes, 1 hour and 2 hours following IPC by ELISA method.

Tissue injury in the mucosa was increased by the duration of preservation time. IPO significantly decreased the intestinal wall injury (p<0.05). Meanwhile, the reperfusion-ended MDA’s value (145±6 vs. 129±3.2 umol/g) was lower; and the capacity and activity of endogenous antioxidant protective systems (GSH: 789±8.2 vs. 834±5.1 umol/g; SOD: 110±9 vs. 126±4.4 IU/g; p<0.05) remained higher in IPO groups. Specific dynamism of NF-kB activation was the follow: total and activated form of NF-kB significantly elevated 30 minutes following IPO. 1 hour after IPO its activation decreased to the control level, however, after 2 hours gradual activation of NF-kB was detected again.

Our findings confirm three cycles of IPO prior to reperfusion could moderate the injury better preserving the endogenous antioxidants and tissue structure. IPO was able to moderate oxidative stress inducing intracellular signalling pathways, which indicated both cytoplasmic and subsequent nuclear NF-kB activation in bowel tissue. (Supported by OTKA PD77474).

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